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MONOCLONAL ANTIBODIES__

 

Monoclonal antibodies to plant cell wall polymers generated or maintained in our laboratory are detailed below. Reference links lead to the most relevant & recent work on epitope characterization.

AVAILABILITY: All the monoclonal antibodies listed here are available for use. To maintain the availability of monoclonal antibodies from our laboratory and support their efficient supply a charging system to cover cell line maintenance and distribution costs is in operation from PlantProbes click here

NOMENCLATURE: LM- Leeds Monoclonal, JIM- John Innes Monoclonal, MAC- Monoclonal Antibody Centre (Babraham).

All antibodies listed here are rat monoclonal antibodies.
Reagents we recommend for their detection include:
Peroxidase-linked

Anti-rat IgG (whole molecule)-peroxidase produced in rabbit (Sigma A9542
)
Fluorochromes
Anti-rat IgG (whole molecule)-FITC produced in rabbit (Sigma F1763)

Gold-linked

Anti-rat IgG (whole molecule)-Gold produced in goat (Sigma G7035)

 
 

Items to download:

List of monoclonal antibodies pdf (2010)

Cartoons: Pectic polymers & antibodies pdf (2003)

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Anti-mannan

LM21       (galacto)(gluco)mannan (Marcus et al. 2010)

LM22       (gluco)mannan (Marcus et al. 2010)

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Anti-xyloglucan

LM15      XXXG motif of xyloglucans (Marcus et al. 2008)

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Anti-xylan

LM10      (1→4)-b-D-xylan (McCartney et al. 2005)

LM11     (14)-b-D-xylan / arabinoxylan (McCartney et al. 2005)

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Anti-pectin

Xylogalacturonan

LM8        xylogalacturonan (Willats et al. 2004)

Homogalacturonan (HG)

LM18    partially Me-HG / de-esterified HG (Verhertbruggen et al. 2008)

LM19    partially Me-HG / de-esterified HG (Verhertbruggen et al. 2008)

LM20    partially Me-HG (Verhertbruggen et al. 2008)

LM7       non-blockwise partially Me-HG, (Willats et al. 2001; Clausen et al. 2003)

PAM1    blockwise de-esterified HG (Willats et al. 1999; Manfield et al., 2005)

JIM5      partially Me-HG / de-esterified HG (Clausen et al. 2003)

JIM7      partially Me-HG (Clausen et al. 2003)

Rhamnogalacturonan-I (RG-I) related   

LM13     linearised (15)-a-L-arabinan (Moller et al. 2008; Verhertbruggen et al. 2009)

LM16     processed arabinan (Verhertbruggen et al. 2009)

LM9       feruloylated (14)-b-D-galactan (Clausen et al. 2004)

LM5       (14)-b-D-galactan (Jones et al. 1997)

LM6*      (15)-a-L-arabinan (Willats et al. 1998)  

*LM6 may also bind to AGPs

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Anti-arabinogalactan-protein (AGP)

LM14         AGP glycan (Moller et al. 2008)
LM2           
b-linked GlcA (Smallwood et al. 1996; Yates et al. 1996)

JIM4           AGP glycan (Knox et al. 1989; Stacey et al. 1990; Yates et al., 1996)

JIM13         AGP glycan (Knox et al. 1991; Yates et al. 1996)

JIM14         AGP glycan (Knox et al. 1991; Yates et al. 1996)

JIM15         AGP glycan (Knox et al. 1991; Yates et al. 1996)

JIM16         AGP glycan (Knox et al. 1991; Yates et al. 1996)

MAC207    AGP glycan (Pennell et al. 1989; Yates et al. 1996)

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Anti-extensin     

LM1            extensin(Smallwood et al. 1995)

JIM11          extensin (Smallwood et al. 1994)

JIM12          extensin (Smallwood et al. 1994)

JIM19         extensin (Smallwood et al. 1994; Wang et al. 1995)

JIM20         extensin (Smallwood et al. 1994; Knox et al. 1995)

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Others    

LM4           pea amine oxidase (Laurenzi et al. 2001)

JIM18        glycophospholipid (Knox et al. 1995; Perotto et al. 1995)

JIM1          b-linked galactose, plasma membrane (Knox & Roberts 1989)

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